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	Provedor de dados Ciência Rural (1) Genet. Mol. Biol. (1) PAB (1) Autor Koehler-Santos,Patrícia (3) Freitas,Loreta Brandão de (2) Ashton-Prolla,Patricia (1) Dornelles,Ana Lúcia Cunha (1) Matte,Ursula (1) Polanczyk,Carisi Anne (1) Salzano,Francisco Mauro (1) Schlatter,Rosane Paixão (1) Mais... Palavra-chave Breeding methods (1) Citrus (1) Cost analysis (1) Defense proteins (1) Genetic variability (1) Genetic variation (1) Hereditary cancer (1) Microsatellites (1) Molecular diagnosis (1) Triticum aestivum L. (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 2003 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses PAB Koehler-Santos,Patrícia; Dornelles,Ana Lúcia Cunha; Freitas,Loreta Brandão de. The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Genetic variation; Microsatellites; Breeding methods. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003 Costs of genetic testing: Supporting Brazilian Public Policies for the incorporating of molecular diagnostic technologies Genet. Mol. Biol. Schlatter,Rosane Paixão; Matte,Ursula; Polanczyk,Carisi Anne; Koehler-Santos,Patrícia; Ashton-Prolla,Patricia. This study identifies and describes the operating costs associated with the molecular diagnosis of diseases, such as hereditary cancer. To approximate the costs associated with these tests, data informed by Standard Operating Procedures for various techniques was collected from hospital software and a survey of market prices. Costs were established for four scenarios of capacity utilization to represent the possibility of suboptimal use in research laboratories. Cost description was based on a single site. The results show that only one technique was not impacted by rising costs due to underutilized capacity. Several common techniques were considerably more expensive at 30% capacity, including polymerase chain reaction (180%), microsatellite instability... Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular diagnosis; Hereditary cancer; Cost analysis. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300332 Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing Ciência Rural Freitas,Loreta Brandão de; Koehler-Santos,Patrícia; Salzano,Francisco Mauro. Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous... Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum L.; Defense proteins; Genetic variability. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016 Registros recuperados: 3 Primeira ... 1 ... Última

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